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KMID : 0359319900300010065
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Korean Journal of Veterinary Research
1990 Volume.30 No. 1 p.65 ~ p.71
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Pathogenicity , hemagglutinability , and the effect of physicochemical agents on virus of rabbit hemorrhagic disease
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Abstract
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Rabbits were experimentally infected with rabbit hemorrhagic disease virus and the viral pathogenicity, hemagglutinability, and the effect of physicochemical factors were studied.
The experimental results were summariaed as follows:
1. Mean rectal temperature of 11 infected rabbits was 40.0±0.47℃ prior to the virus inoculation, and 39.9±0.75℃ after 12hrs., 40.2±0.65℃ after 24hrs., 40.1±0.77℃ after 36hurs, and 40.6±0.56℃ just before the death.
2. Mean death time of infected rabbits was 40. 3±22.0 honrs and its range was 24 to 93 hours.
3. O, B, AB and A type of human erythrocytes were shown their HA in the order, but rabbit and chicken erythrocytes were not hemagglutinated by the virus.
4. In the hemagglutination, less than 0.25 per cent of a final concentration of erythrocytes and 0.2 per cent of BSA in PBS resulted in the best hemagglutination. Phosphate concentration in a range of 0.01M to 0.l0M in PBS was not influenced on the hemagglutination reaction, and its pH 7.0 resulted in a better HA.
5. The hemagglutinating titers, in log 2 scale, of organs and tissues of the virus infected rabbits were 9.3±3.8 (liver), 9.1±3.9 (blood), 6.2±2.6 (spleen) and 5.0±2.5 (kidney).
6. The physicochemical factors such as heating (50℃, 10 min.), trypsin treatment (0.05 pre cent, 5 min.), acid treatment (pH 3.0, 20 min.) and ether extraction (3 times) were not affective to the stability of virus and viral HA activities.
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